The perennial medicinal climber Cayratia trifolia (Family: Vitaceae) is well known throughout Asia and Australia for its ethnomedical uses. In the current study, secondary metabolites were qualitatively screened for in the ethanolic extracts of the plant parts. Alkaloids, phenols, tannins, flavonoids, saponins, terpenoids, and glycosides were consistently found in all organs under investigation, according to the analysis. According to phytochemical profiling, the leaves and stems are especially abundant in stilbenes like resveratrol, piceid, viniferin, and ampelopsin, as well as flavonoids like kaempferol, quercetin, myricetin, cyanidin, and delphinidin. Roots and aerial parts were consistently found to contain tannins, steroids, and terpenoids, including epifriedelanol. In all ethanolic extracts, alkaloids and glycosides were also detected using conventional reagent assays. These results support earlier research showing a wide range of phyto-constituents in C. trifolia, which is consistent with its known pharmacological properties, including anti-inflammatory, anti-bacterial, antiprotozoal, anticancer, antidiabetic, and antidiuretic effects. Significant bioactive potential is suggested by the presence of these various secondary metabolites in ethanolic extracts. While alkaloids, saponins, and terpenoids, such as epifriedelanol, are responsible for antimicrobial, cytotoxic, and enzyme-inhibitory effects, flavonoids and tannins help to scavenge free radicals and reduce inflammation. Cayratia trifolia concludes by confirming that all tested portions contain significant amounts of the important secondary metabolites alkaloids, phenols & tannins, flavonoids, saponins, terpenoids, and glycosides. The plant's widespread traditional medicinal use is supported by the distribution of these compounds, which also supports additional bioactivity-guided research aimed at identifying, describing, and assessing these compounds for potential therapeutic uses.