In the verdant landscapes of Assam, the Kaji Nemu (Assam Lemon) not only represents a cherished cultural emblem but also stands as a testament to the region’s agro-biodiversity. The conferment of the Geographical Indication (GI) tag in the year 2019 on this citrus variety marks a pivotal moment, intertwining the preservation of agricultural traditions with the propulsion of local agribusiness into the global marketplace. This paper delves into the dual role of GI tags as guardians of authenticity, safeguarding indigenous horticultural practices while simultaneously catalyzing economic growth for Assam’s agrarian communities. Through an analytical lens, the study examines the synergistic impact of India’s Protection of Plant Varieties and Farmers’ Rights Act, 2001 and the Seeds Act, 1966 on the Assam Lemon’s journey from local orchards to international markets. It highlights how these legislative frameworks underpin the GI tag’s efficacy in enhancing market differentiation, ensuring quality and protecting farmers’ rights. Furthermore, the paper explores the opportunities that GI tags unlock for farmers including brand recognition, legal protection and government support which collectively contribute to elevating Assam Lemon to a symbol of rural prosperity. The research culminates in strategic recommendations aimed at amplifying the reach of GI-tagged Assam Lemon. These include leveraging policy initiatives, fostering public-private partnerships and harnessing innovative marketing strategies to bridge the gap between local excellence and global demand. The paper underscores the transformative potential of GI tags in fortifying Assam’s agribusiness heralding a new era of sustainable development rooted in the authenticity of its agricultural heritage.