Plant tissue culture methods were applied for micropropagation of Anogeissus latifolia, Caraluma edulis, Ceropegia bulbosa and Withania coagulans. These are ecologically and economically valuable plant species of fragile ecosystems of the Aravalis and the Indian desert. Micropropagation protocol was developed for Anogeissus acuminata – a tree of the Aravalis. Cotyledonary node explants were cultured on MS + 2.0 mgl-1 BAP + 0.1 mgl-1 NAA and other additives. The shoots were multiplied for large scale plant regeneration. These were rooted on half strength MS salts + 0.1 mgl-1 IBA + 100 mgl-1 activated charcoal. Tissue culture processes were also defined for C. edulis and C. bulbosa. In case of withania coagulans cultures were established and multiplied on modified MS medium. Plantlets generated through tissue culture were hardened in green house and transferred to pots/polybags. The procedure developed can be useful for large scale production of these plants for afforestation and also for the conservation of germplasm.

Keywords: Anogenissis Acuminata, Ceropegia Bulbosa, Withania Coagulans, Micropropagation.