Swiss albino mice were treated with Lead acetate (20 mg/kg wt.) in the presence (experimental) or absence (control) of both vitamins α-tocopherol (Vitamin E) 25 mg/kg and Ascorbic acid (Vitamin C) 500 mg/kg body weight. Animals were autopsied and their livers were removed at various intervals from 6 hrs to 20 days. The percentage of normal hepatocytes in both groups gradually decreased until day 2, but increased rapidly thereafter, reaching near normal values at the final autopsy interval (i.e., day 20) in the experimental group. In contrast, binucleate hepatocytes increased by day 2, then gradually decreased, returning to normal levels by day 10 in animals treated with Vitamin E + Vitamin C. The frequency of abnormal cells also increased by day 5, but decreased without reaching normal levels by the end of experiment. The counts of such cells declined significantly in mice, who received Vitamins before lead intoxication. It is concluded that Vitamin E & C can be used as preventative to inhabit lead-induced hepatic lesions in mice.

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Keywords: Lead Acetate, α-Tocopherol, Ascorbic Acid, Hepatocytes, Binucleate.