Plants are very important part of mankind from history these are used in various kind of medicines. There are different kind of medicinal plants which are in use from years to treat infections and diseases.  This study shows the antimicrobial activity of Neem (Azadirachta indica) which was evaluated against two gram-negative bacteria Escherichia coli and Salmonella typhi (pathogenic bacteria) and gram-positive bacteria (Bacillus subtilis). Azadirachta indica green leaf and bark was collected from the local fields of Medicinal Garden in School of Pharmaceutical Studies, Faculty of Heath Sciences, Newai and clear cultures of the test micro-organisms used for antimicrobial study were provided by the Dr. K. N. Modi University, Newai Rajasthan. All the test microorganisms were selected for their antibacterial activity with leaf and bark extract of Azadirachta indica by agar well diffusion method. Leaf and bark extracts of Azadirachta indica showed good inhibition zone against the gram-positive and gram-negative bacteria.

Keywords: Azadirachta Indica, Antimicrobial Activity, Pathogenic Bacteria, Leaf and Bark Extract.