Alkaline proteases have high commercial value and find multiple applications in various industrial sectors. The present study intended to isolate a suitable bacterium for alkaline protease production. Protease producing bacteria were isolated from organic waste containing soil, screened for protease production on skim milk agar plates and confirmed the protease production through protease assay. In Study Area Rajasthan of India, almost 50% of the territory presents a predominant arid climate that includes alluvial origin principally in the arid regions, located mainly in the north of the state. The soils in Rajasthan state are typical of saline soils of the arid region of India, with the presence of alluvial and lacustrine deposits, some of them calcareous or gypsiferous. In present research study with the aim of isolating halophilic bacteria, three geographical areas of Jaipur District in Rajasthan were selected for study and were sampled (Figure 1); the areas were selected according to their saline or alkaline-sodic characteristics. In the present study, soil samples collected from Jaipur were initially analysed for Quality controls including Microbiological lab and product development. Samples were serially diluted using distilled water and spread plated on the surface of casein agar plates (nutrient agar with 1% casein) and incubated at 30oC for 48hrs.
Keywords: Alkaline Protease, Fermentation, Protease assay, Bacillus Cereus, Protease Production.
